Standard Operating Procedure (SOP) for Operating Procedure of Double Door Autoclave

Cleaning of High Performance Liquid Chromatography (HPLC)

1. Purpose of Operating Procedure of Double Door Autoclave

The purpose of this Standard Operating Procedure (SOP) is to provide clear guidelines for the proper operation and utilization of the double door autoclave within [Company Name]. This SOP aims to ensure the safe and effective use of the autoclave for sterilization purposes.

2. Scope of Operating Procedure of Double Door Autoclave

This SOP applies to all personnel responsible for operating and utilizing the double door autoclave within [Company Name]. It encompasses the startup, operation, and shutdown procedures for the autoclave.

3. Definitions involved in Operating Procedure of Double Door Autoclave

3.1 Double Door Autoclave:

A specialized autoclave with two doors, allowing for separate loading and unloading of materials in a controlled and sterile environment.

3.2 Sterilization:

The process of eliminating or killing all microorganisms, including spores and viruses, from materials or equipment.

3.3 Chamber:

The internal compartment of the autoclave where materials to be sterilized are placed.

4. Safety Precautions and Personal Protective Equipment (PPE)

4.1 PPE Requirements

4.1.1 Before operating the double door autoclave, personnel shall wear the required PPE, including lab coats, gloves, safety goggles, and closed-toe shoes.

4.1.2 PPE shall be properly inspected for any damage or wear before use.

4.2 Chamber Inspection

4.2.1 Visually inspect the autoclave chamber for cleanliness, any visible residues, or foreign materials.

4.2.2 Ensure that the chamber is free from any potential obstructions that may hinder the sterilization process.

5. Startup and Loading

5.1 Power On

5.1.1 Turn on the power to the autoclave using the designated control panel.

5.1.2 Allow the autoclave to complete its startup sequence and stabilize.

5.2 Loading Materials

5.2.1 Open one of the autoclave doors and load the materials to be sterilized into the chamber.

5.2.2 Follow proper loading procedures to ensure even distribution of materials and efficient sterilization.

5.3 Door Closure and Sealing

5.3.1 Carefully close and secure both doors of the autoclave.

5.3.2 Ensure that the doors are properly sealed to maintain a sterile environment during the sterilization process.

6. Sterilization Cycle

6.1 Program Selection

6.1.1 Select the appropriate sterilization program on the autoclave control panel based on the type of materials being sterilized.

6.1.2 Ensure that the selected program aligns with the manufacturer’s guidelines and the nature of the materials.

6.2 Parameters Input

6.2.1 Input the required parameters, such as temperature, pressure, and cycle duration, as per the sterilization program.

6.2.2 Verify the input parameters before initiating the sterilization cycle.

6.3 Cycle Initiation

6.3.1 Start the sterilization cycle using the autoclave control panel.

6.3.2 Monitor the cycle progress and any alerts or alarms displayed on the panel.

7. Unloading and Shutdown

7.1. Cycle Completion

7.1.1 Wait for the autoclave to complete the sterilization cycle as indicated on the control panel.

7.1.2 Ensure that any required safety interlocks are released before proceeding.

7.2 Door Opening

7.2.1 Carefully open the autoclave doors after ensuring that the chamber pressure has equalized.

7.2.2 Use caution when opening the doors to prevent steam exposure.

7.3 Unloading Materials

7.3.1 Remove the sterilized materials from the autoclave chamber using sterile techniques.

7.3.2 Transfer the materials to a designated area for cooling and further processing.

7.4 Power Off

7.4.1 Turn off the power to the autoclave using the designated control panel.

7.4.2 Ensure that the autoclave is safely powered down before leaving the area.

8. Documentation and Recordkeeping

8.1. Cycle Log

8.1.1 Record details of each sterilization cycle, including date, time, cycle parameters, materials sterilized, and any observations.

8.1.2 Ensure that the cycle log is signed and dated.

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Pharmacareer team is a team of Experts from every department of Pharmaceutical industry having enriched experience. Experts have work experience of many multinational pharmaceutical industries worldwide.

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