Standard Operating Procedure (SOP) for Calibration Procedure of UV Cabinet

Standard Operating Procedure (SOP) for Calibration of Conductivity/TDS Meter

1. Purpose of Calibration Procedure of UV Cabinet

The purpose of this Standard Operating Procedure (SOP) for Calibration Procedure of UV Cabinet is to establish a systematic process for calibrating the UV (Ultraviolet) cabinet within [Company Name]. This procedure ensures the accuracy and reliability of UV radiation levels for various applications.

2. Scope of Calibration Procedure of UV Cabinet

This SOP for Calibration Procedure of UV Cabinet applies to all personnel responsible for operating and maintaining the UV cabinet within [Company Name]. It encompasses the procedures for calibration setup, UV lamp performance verification, and documentation.

3. Definitions involved in Calibration Procedure of UV Cabinet

3.1 UV Cabinet:

An enclosed workspace equipped with UV lamps used for various applications, such as DNA visualization, bacterial sterilization, or fluorescence studies.

3.2 Calibration:

The process of adjusting and verifying the accuracy of the UV lamp output.

4. Safety Precautions and Personal Protective Equipment (PPE)

4.1. PPE Requirements

4.1.1 Personnel conducting UV cabinet calibration must wear appropriate PPE, including lab coats, gloves, safety goggles, and face shields when working with UV radiation.

4.1.2 Ensure that PPE is in good condition and properly fitted.

4.2. UV Radiation Safety

4.2.1 Avoid direct exposure to UV radiation. Keep the UV cabinet door closed during operation.

4.2.2 Use caution when handling UV lamps and UV-sensitive materials.

5. Calibration Setup

5.1. Equipment and Materials

5.1.1 Gather the necessary equipment, including a calibrated radiometer, a UV intensity meter, and UV dosimeters.

5.1.2 Ensure that all equipment is clean, in good working condition, and calibrated.

5.2. UV Lamp Warm-Up

5.2.1 Turn on the UV cabinet and allow the UV lamps to warm up for at least 15 minutes before calibration.

5.2.2 Ensure that the cabinet is empty during warm-up.

6. Calibration Procedure

6.1. Radiometer Calibration

6.1.1 Calibrate the radiometer following the manufacturer’s instructions or using a certified calibration source.

6.1.2 Record the calibration details, including date and calibration source used.

6.2. UV Lamp Intensity Measurement

6.2.1 Position the radiometer or UV intensity meter at the center of the UV cabinet workspace.

6.2.2 Measure the UV lamp intensity at different positions inside the cabinet to ensure uniformity.

6.3. UV Dosimeter Placement

6.3.1 Place UV dosimeters at various locations inside the cabinet, including near the UV lamps and at typical sample positions.

6.3.2 Record the dosimeter placement locations.

6.4. UV Lamp Adjustment

6.4.1 If variations in UV lamp intensity are observed, adjust the lamp position or replace lamps as needed.

6.4.2 Repeat the UV intensity measurements and dosimeter readings until uniform UV radiation levels are achieved.

7. Calibration Verification

7.1. Dosimeter Reading

7.1.1 Retrieve the dosimeters from their placement locations.

7.1.2 Record the dosimeter readings and compare them to the desired UV radiation levels.

7.2. Documentation of Calibration

7.2.1 Maintain detailed records of the calibration session, including date, time, equipment used, UV intensity measurements, dosimeter readings, and any adjustments made.

7.2.2 Ensure that all records are signed, dated, and readily accessible for review.

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