{"id":5899,"date":"2023-10-19T19:26:01","date_gmt":"2023-10-19T13:56:01","guid":{"rendered":"https:\/\/pharmaceuticalcarrier.com\/?p=5899"},"modified":"2023-10-19T19:26:12","modified_gmt":"2023-10-19T13:56:12","slug":"standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system","status":"publish","type":"post","link":"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/","title":{"rendered":"Standard Operating Procedure (SOP) for Safely Starting and Shutting Down an HPLC System"},"content":{"rendered":"\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_76 counter-hierarchy ez-toc-counter ez-toc-transparent ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#1_Purpose_of_Safely_Starting_and_Shutting_Down_an_HPLC_System\" >1. Purpose of Safely Starting and Shutting Down an HPLC System<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#2_Scope_of_Safely_Starting_and_Shutting_Down_an_HPLC_System\" >2. Scope of Safely Starting and Shutting Down an HPLC System<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#3_Definitions\" >3. Definitions<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#31_HPLC_System\" >3.1 HPLC System:<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#4_Responsibilities\" >4. Responsibilities<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#41_Laboratory_Manager\" >4.1. Laboratory Manager<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#42_Analysts\" >4.2. Analysts<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#5_HPLC_System_Start-Up\" >5. HPLC System Start-Up<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#51_Preliminary_Checks\" >5.1. Preliminary Checks<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#52_Powering_On_the_System\" >5.2. Powering On the System<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-11\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#53_System_Initialization\" >5.3. System Initialization<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-12\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#54_Preliminary_Set-Up\" >5.4. Preliminary Set-Up<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-13\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#6_HPLC_System_Shutdown\" >6. HPLC System Shutdown<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-14\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#61_Completing_the_Analysis\" >6.1. Completing the Analysis<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-15\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#62_Shutting_Down_the_System\" >6.2. Shutting Down the System<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-16\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2023\/10\/19\/standard-operating-procedure-sop-for-safely-starting-and-shutting-down-an-hplc-system\/#63_Post-Analysis_Checks\" >6.3. Post-Analysis Checks<\/a><\/li><\/ul><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"1_Purpose_of_Safely_Starting_and_Shutting_Down_an_HPLC_System\"><\/span><strong>1. Purpose of Safely Starting and Shutting Down an HPLC System<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>The purpose of this Standard Operating Procedure (SOP) is to provide step-by-step instructions for the safe and effective start-up and shutdown of the High-Performance Liquid Chromatography (HPLC) system at [Company Name]. This procedure is designed to ensure the proper operation of the HPLC system and maintain the safety of laboratory personnel.<\/p><div class=\"pharm-content\" id=\"pharm-3873222883\"><script async src=\"\/\/pagead2.googlesyndication.com\/pagead\/js\/adsbygoogle.js?client=ca-pub-8402705006112223\" crossorigin=\"anonymous\"><\/script><ins class=\"adsbygoogle\" style=\"display:block;\" data-ad-client=\"ca-pub-8402705006112223\" \ndata-ad-slot=\"7652789694\" \ndata-ad-format=\"auto\"><\/ins>\n<script> \n(adsbygoogle = window.adsbygoogle || []).push({}); \n<\/script>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"2_Scope_of_Safely_Starting_and_Shutting_Down_an_HPLC_System\"><\/span><strong>2. Scope of Safely Starting and Shutting Down an HPLC System<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>This SOP applies to all laboratory personnel responsible for operating the HPLC system at [Company Name]. It covers the entire process, from initial start-up to system shutdown.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"3_Definitions\"><\/span><strong>3. Definitions<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"31_HPLC_System\"><\/span>3.1 <strong>HPLC System<\/strong>: <span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>High-Performance Liquid Chromatography system, which is used for separating, identifying, and quantifying analytes in a sample.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"4_Responsibilities\"><\/span><strong>4. Responsibilities<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"41_Laboratory_Manager\"><\/span><strong>4.1. Laboratory Manager<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>4.1.1 Ensure that laboratory personnel are trained and competent in HPLC system start-up and shutdown procedures.<\/p>\n\n\n\n<p>4.1.2 Oversee compliance with this SOP.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"42_Analysts\"><\/span><strong>4.2. Analysts<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>4.2.1 Follow this SOP for HPLC system start-up and shutdown.<\/p>\n\n\n\n<p>4.2.2 Report any deviations or issues to the Laboratory Manager.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"5_HPLC_System_Start-Up\"><\/span><strong>5. HPLC System Start-Up<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"51_Preliminary_Checks\"><\/span><strong>5.1. Preliminary Checks<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>5.1.1 Inspect the HPLC system for any visible damage or abnormalities.<\/p>\n\n\n\n<p>5.1.2 Verify that all necessary components (columns, detectors, etc.) are properly installed.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"52_Powering_On_the_System\"><\/span><strong>5.2. Powering On the System<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>5.2.1 Turn on the main power source of the HPLC system.<\/p>\n\n\n\n<p>5.2.2 Power on the computer and related software, if applicable.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"53_System_Initialization\"><\/span><strong>5.3. System Initialization<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>5.3.1 Allow the HPLC system to initialize. This may involve an automatic system check.<\/p>\n\n\n\n<p>5.3.2 Verify that the system software is properly loaded and operational.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"54_Preliminary_Set-Up\"><\/span><strong>5.4. Preliminary Set-Up<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>5.4.1 Ensure that the mobile phase and sample vials are properly labeled and ready for use.<\/p>\n\n\n\n<p>5.4.2 Perform any necessary column equilibration or conditioning, following method-specific instructions.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"6_HPLC_System_Shutdown\"><\/span><strong>6. HPLC System Shutdown<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"61_Completing_the_Analysis\"><\/span><strong>6.1. Completing the Analysis<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>6.1.1 Finish the analysis as per the established chromatographic method.<\/p>\n\n\n\n<p>6.1.2 Ensure that all data is saved properly.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"62_Shutting_Down_the_System\"><\/span><strong>6.2. Shutting Down the System<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>6.2.1 Stop the flow of the mobile phase.<\/p>\n\n\n\n<p>6.2.2 Power off the HPLC system.<\/p>\n\n\n\n<p>6.2.3 Properly store any columns or equipment not in use.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"63_Post-Analysis_Checks\"><\/span><strong>6.3. Post-Analysis Checks<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<p>6.3.1 Verify that all waste containers are properly labeled and ready for disposal.<\/p>\n\n\n\n<p>6.3.2 Clean the system, including sample injection ports and detectors, following established procedures.<\/p>\n\n\n\n<p>For more articles,&nbsp;<strong><a href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/ayurveda\/\">Kindly Click<\/a>&nbsp;here<\/strong>.<\/p>\n\n\n\n<p>For pharmaceutical jobs, follow us on<strong>&nbsp;<a href=\"https:\/\/www.linkedin.com\/company\/pharmaceuticalcarrier\/?viewAsMember=true\" target=\"_blank\" rel=\"noreferrer noopener\">LinkedIn<\/a><\/strong>&nbsp;<\/p>\n\n\n\n<p><strong>For Editable SOPs in word format contact us on&nbsp;<a href=\"mailto:info@pharmaceuticalcarrier.com\">info@pharmaceuticalcarrier.com<\/a><\/strong>&nbsp;<\/p>\n\n\n\n<p>For more information kindly follow us on pharmaguidelines.co.uk<\/p>\n","protected":false},"excerpt":{"rendered":"<p>This Standard Operating Procedure (SOP) outlines the step-by-step instructions for safely starting and shutting down an HPLC system. It ensures the proper operation of the High-Performance Liquid Chromatography (HPLC) system and the safety of laboratory personnel. Explore this comprehensive SOP to maintain laboratory safety and quality standards.<\/p>\n","protected":false},"author":1,"featured_media":5599,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[165],"tags":[815,843,729,885,883,677,346,884,166,167,886],"class_list":["post-5899","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-standard-operating-procedure","tag-analytical-chemistry","tag-equipment-operation","tag-hplc","tag-hplc-system","tag-laboratory-operations","tag-laboratory-procedures","tag-laboratory-safety","tag-shutdown-procedure","tag-sop","tag-standard-operating-procedure","tag-start-up-procedure"],"uagb_featured_image_src":{"full":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure.jpg",1200,720,false],"thumbnail":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure-150x150.jpg",150,150,true],"medium":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure-300x180.jpg",300,180,true],"medium_large":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure-768x461.jpg",640,384,true],"large":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure-1024x614.jpg",640,384,true],"1536x1536":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure.jpg",1200,720,false],"2048x2048":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure.jpg",1200,720,false],"authorship-box-avatar":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure-150x150.jpg",150,150,true],"authorship-box-related":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure-70x70.jpg",70,70,true],"sow-carousel-default":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure.jpg",272,163,false]},"uagb_author_info":{"display_name":"Pharmacareer","author_link":"https:\/\/pharmaceuticalcarrier.com\/index.php\/author\/grandirpharmagmail-com\/"},"uagb_comment_info":0,"uagb_excerpt":"This Standard Operating Procedure (SOP) outlines the step-by-step instructions for safely starting and shutting down an HPLC system. It ensures the proper operation of the High-Performance Liquid Chromatography (HPLC) system and the safety of laboratory personnel. Explore this comprehensive SOP to maintain laboratory safety and quality standards.","amp_enabled":true,"_links":{"self":[{"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/posts\/5899","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/comments?post=5899"}],"version-history":[{"count":1,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/posts\/5899\/revisions"}],"predecessor-version":[{"id":5900,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/posts\/5899\/revisions\/5900"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/media\/5599"}],"wp:attachment":[{"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/media?parent=5899"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/categories?post=5899"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/tags?post=5899"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}