{"id":7084,"date":"2024-04-22T18:27:13","date_gmt":"2024-04-22T12:57:13","guid":{"rendered":"https:\/\/pharmaceuticalcarrier.com\/?p=7084"},"modified":"2024-04-11T16:11:39","modified_gmt":"2024-04-11T10:41:39","slug":"standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph","status":"publish","type":"post","link":"https:\/\/pharmaceuticalcarrier.com\/index.php\/2024\/04\/22\/standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph\/","title":{"rendered":"Standard Operating Procedure (SOP) for Calibration of HPLC (High-Performance Liquid Chromatograph)"},"content":{"rendered":"\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_76 counter-hierarchy ez-toc-counter ez-toc-transparent ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2024\/04\/22\/standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph\/#10_Purpose\" >1.0 Purpose:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2024\/04\/22\/standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph\/#20_Scope\" >2.0 Scope:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2024\/04\/22\/standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph\/#30_Responsibilities\" >3.0 Responsibilities:<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2024\/04\/22\/standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph\/#31_The_Analyst_%E2%80%93\" >3.1 The Analyst &#8211;<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2024\/04\/22\/standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph\/#40_Equipment_and_Materials\" >4.0 Equipment and Materials:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2024\/04\/22\/standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph\/#50_Procedure\" >5.0 Procedure:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2024\/04\/22\/standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph\/#60_Documentation\" >6.0 Documentation:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2024\/04\/22\/standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph\/#70_Training\" >7.0 Training:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2024\/04\/22\/standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph\/#80_References\" >8.0 References:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2024\/04\/22\/standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph\/#90_Revision_History\" >9.0 Revision History:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-11\" href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/2024\/04\/22\/standard-operating-procedure-sop-for-calibration-of-hplc-high-performance-liquid-chromatograph\/#100_Approval\" >10.0 Approval:<\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"10_Purpose\"><\/span><strong>1.0 Purpose:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>1.1 This Standard Operating Procedure (SOP) outlines the systematic procedure for the calibration of High-Performance Liquid Chromatography (HPLC) equipment, specifically the Low-Pressure Quaternary Gradient Liquid Chromatograph, to ensure accurate and reliable analytical results.<\/p><div class=\"pharm-content\" id=\"pharm-1370437582\"><script async src=\"\/\/pagead2.googlesyndication.com\/pagead\/js\/adsbygoogle.js?client=ca-pub-8402705006112223\" crossorigin=\"anonymous\"><\/script><ins class=\"adsbygoogle\" style=\"display:block;\" data-ad-client=\"ca-pub-8402705006112223\" \ndata-ad-slot=\"7652789694\" \ndata-ad-format=\"auto\"><\/ins>\n<script> \n(adsbygoogle = window.adsbygoogle || []).push({}); \n<\/script>\n<\/div>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"20_Scope\"><\/span><strong>2.0 Scope:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>2.1 This procedure is applicable to all HPLC instruments utilizing a Low-Pressure Quaternary Gradient system within the [Your Company Name] laboratory.<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"30_Responsibilities\"><\/span><strong>3.0 Responsibilities:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"31_The_Analyst_%E2%80%93\"><\/span>3.1 The Analyst &#8211; <span class=\"ez-toc-section-end\"><\/span><\/h3>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"40_Equipment_and_Materials\"><\/span><strong>4.0 Equipment and Materials:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>4.1 HPLC instrument with a Low-Pressure Quaternary Gradient system.<\/p>\n\n\n\n<p>4.2 Certified standard solutions for calibration.<\/p>\n\n\n\n<p>4.3 Graduated cylinders.<\/p>\n\n\n\n<p>4.4 Analytical balance.<\/p>\n\n\n\n<p>4.5 Chromatographic columns.<\/p>\n\n\n\n<p>4.6 Mobile phase solutions.<\/p>\n\n\n\n<p>4.7 Injection syringes.<\/p>\n\n\n\n<p>4.8 HPLC-compatible solvent.<\/p>\n\n\n\n<p>4.9 Calibration report template.<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"50_Procedure\"><\/span><strong>5.0 Procedure:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p><strong>5.1 Preliminary Checks:<\/strong><\/p>\n\n\n\n<p>5.1.1 Ensure the HPLC instrument is clean and free from any contamination.<\/p>\n\n\n\n<p>5.1.2 Verify that the instrument is in the standby mode and is properly connected to the power source.<\/p>\n\n\n\n<p>5.1.3 Check the condition of the chromatographic column and replace if necessary.<\/p>\n\n\n\n<p><strong>5.2 Instrument Warm-up:<\/strong><\/p>\n\n\n\n<p>5.2.1 Turn on the HPLC instrument and allow it to warm up for a minimum of 30 minutes.<\/p>\n\n\n\n<p><strong>5.3 System Suitability Test:<\/strong><\/p>\n\n\n\n<p>5.3.1 Run a system suitability test using a standard solution to ensure the proper functioning of the HPLC system.<\/p>\n\n\n\n<p><strong>5.4 Calibration of Detectors:<\/strong><\/p>\n\n\n\n<p>5.4.1 Calibrate the UV\/Visible and other detectors according to the manufacturer&#8217;s specifications.<\/p>\n\n\n\n<p><strong>5.5 Calibration of Pumps:<\/strong><\/p>\n\n\n\n<p>5.5.1 Prime the pumps with the HPLC-compatible solvent.<\/p>\n\n\n\n<p>5.5.2 Calibrate the flow rate and pressure using certified standard solutions.<\/p>\n\n\n\n<p><strong>5.6 Calibration of Injection System:<\/strong><\/p>\n\n\n\n<p>5.6.1 Load the injection syringe with a known concentration of the standard solution.<\/p>\n\n\n\n<p>5.6.2 Inject the standard solution and ensure proper peak shape and retention time.<\/p>\n\n\n\n<p><strong>5.7 Calibration of Gradient System:<\/strong><\/p>\n\n\n\n<p>5.7.1 Prepare a set of standard solutions covering the desired concentration range.<\/p>\n\n\n\n<p>5.7.2 Run a gradient program using the standard solutions and verify the linearity of the detector response.<\/p>\n\n\n\n<p><strong>5.8 Data Analysis:<\/strong><\/p>\n\n\n\n<p>5.8.1 Analyze the chromatograms obtained during the calibration for peak resolution, symmetry, and accuracy.<\/p>\n\n\n\n<p>5.8.2 Document the results in the calibration report template.<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"60_Documentation\"><\/span><strong>6.0 Documentation:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>This Standard Operating Procedure (SOP) outlines the specific guidelines for the comprehensive documentation of the High-Performance Liquid Chromatography (HPLC) equipment calibration process, emphasizing the Low-Pressure Quaternary Gradient Liquid Chromatograph system.<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"70_Training\"><\/span><strong>7.0 Training:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>7.1 Ensure that all personnel involved in HPLC calibration are adequately trained and understand this SOP.<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"80_References\"><\/span><strong>8.0 References:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>8.1 Manufacturer&#8217;s manuals for HPLC instrument and components.<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"90_Revision_History\"><\/span><strong>9.0 Revision History:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<figure class=\"wp-block-table\"><table><thead><tr><th>Revision No.<\/th><th>Description<\/th><th>Date<\/th><\/tr><\/thead><tbody><tr><td>1.0<\/td><td>Initial release<\/td><td>[Date]<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"100_Approval\"><\/span><strong>10.0 Approval:<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>10.1 [Name and Signature of Approving Authority]<\/p>\n\n\n\n<p>For more articles,&nbsp;<strong><a href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/ayurveda\/\">Kindly Click<\/a>&nbsp;here<\/strong>.<\/p>\n\n\n\n<p>For pharmaceutical&nbsp;<a href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/jobs\/\">jobs<\/a>, follow us on<strong>&nbsp;<a href=\"https:\/\/www.linkedin.com\/company\/pharmaceuticalcarrier\/?viewAsMember=true\" target=\"_blank\" rel=\"noreferrer noopener\">LinkedIn<\/a><\/strong>&nbsp;<\/p>\n\n\n\n<p><strong>For Editable SOPs in Word format&nbsp;<a href=\"https:\/\/pharmaceuticalcarrier.com\/index.php\/contact\/\">contact us<\/a>&nbsp;on&nbsp;<a href=\"mailto:info@pharmaceuticalcarrier.com\">info@pharmaceuticalcarrier.com<\/a><\/strong>&nbsp;<\/p>\n\n\n\n<p>For more information kindly follow us on <a href=\"https:\/\/pharmaguidelines.co.uk\/\">www.pharmaguidelines.co.uk<\/a><\/p>\n\n\n\n<p>Download Above SOP<\/p>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-fdadc9a7-01a3-4974-bcc0-d88675e68ac3\" href=\"https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2024\/03\/SOP-for-Download-Calibration-of-HPLC.docx\">SOP-for-Download-Calibration-of-HPLC<\/a><a href=\"https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2024\/03\/SOP-for-Download-Calibration-of-HPLC.docx\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-fdadc9a7-01a3-4974-bcc0-d88675e68ac3\">Download<\/a><\/div>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n","protected":false},"excerpt":{"rendered":"<p>&#8220;Discover precision and reliability with our High-Performance Liquid Chromatography (HPLC) calibration services. Our expert team ensures accurate results through a systematic calibration process, adhering to industry standards. Trust us for meticulous HPLC instrument calibration, providing you with confidence in your analytical outcomes.&#8221;<\/p>\n","protected":false},"author":1,"featured_media":5599,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_uag_custom_page_level_css":"","footnotes":""},"categories":[156,165],"tags":[1623,1616,1622,1617,1627,1625,411,1626,1621,729,1620,654,1619,875,1618,1624,256],"class_list":["post-7084","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-pharmaceutical-industry","category-standard-operating-procedure","tag-analytical-accuracy","tag-analytical-instruments","tag-calibration-report","tag-calibration-services","tag-chromatogram-analysis","tag-chromatographic-columns","tag-data-analysis","tag-detector-calibration","tag-gradient-system-calibration","tag-hplc","tag-injection-system-calibration","tag-laboratory-equipment","tag-laboratory-standards","tag-liquid-chromatography","tag-precision-calibration","tag-pump-calibration","tag-quality-assurance"],"uagb_featured_image_src":{"full":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure.jpg",1200,720,false],"thumbnail":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure-150x150.jpg",150,150,true],"medium":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure-300x180.jpg",300,180,true],"medium_large":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure-768x461.jpg",640,384,true],"large":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure-1024x614.jpg",640,384,true],"1536x1536":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure.jpg",1200,720,false],"2048x2048":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure.jpg",1200,720,false],"authorship-box-avatar":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure-150x150.jpg",150,150,true],"authorship-box-related":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure-70x70.jpg",70,70,true],"sow-carousel-default":["https:\/\/pharmaceuticalcarrier.com\/wp-content\/uploads\/2023\/09\/Standard-operating-procedure.jpg",272,163,false]},"uagb_author_info":{"display_name":"Pharmacareer","author_link":"https:\/\/pharmaceuticalcarrier.com\/index.php\/author\/grandirpharmagmail-com\/"},"uagb_comment_info":160,"uagb_excerpt":"\"Discover precision and reliability with our High-Performance Liquid Chromatography (HPLC) calibration services. Our expert team ensures accurate results through a systematic calibration process, adhering to industry standards. Trust us for meticulous HPLC instrument calibration, providing you with confidence in your analytical outcomes.\"","amp_enabled":true,"_links":{"self":[{"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/posts\/7084","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/comments?post=7084"}],"version-history":[{"count":5,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/posts\/7084\/revisions"}],"predecessor-version":[{"id":7325,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/posts\/7084\/revisions\/7325"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/media\/5599"}],"wp:attachment":[{"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/media?parent=7084"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/categories?post=7084"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/pharmaceuticalcarrier.com\/index.php\/wp-json\/wp\/v2\/tags?post=7084"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}