1. Purpose of Safely Starting and Shutting Down an HPLC System
The purpose of this Standard Operating Procedure (SOP) is to provide step-by-step instructions for the safe and effective start-up and shutdown of the High-Performance Liquid Chromatography (HPLC) system at [Company Name]. This procedure is designed to ensure the proper operation of the HPLC system and maintain the safety of laboratory personnel.
2. Scope of Safely Starting and Shutting Down an HPLC System
This SOP applies to all laboratory personnel responsible for operating the HPLC system at [Company Name]. It covers the entire process, from initial start-up to system shutdown.
3. Definitions
3.1 HPLC System:
High-Performance Liquid Chromatography system, which is used for separating, identifying, and quantifying analytes in a sample.
4. Responsibilities
4.1. Laboratory Manager
4.1.1 Ensure that laboratory personnel are trained and competent in HPLC system start-up and shutdown procedures.
4.1.2 Oversee compliance with this SOP.
4.2. Analysts
4.2.1 Follow this SOP for HPLC system start-up and shutdown.
4.2.2 Report any deviations or issues to the Laboratory Manager.
5. HPLC System Start-Up
5.1. Preliminary Checks
5.1.1 Inspect the HPLC system for any visible damage or abnormalities.
5.1.2 Verify that all necessary components (columns, detectors, etc.) are properly installed.
5.2. Powering On the System
5.2.1 Turn on the main power source of the HPLC system.
5.2.2 Power on the computer and related software, if applicable.
5.3. System Initialization
5.3.1 Allow the HPLC system to initialize. This may involve an automatic system check.
5.3.2 Verify that the system software is properly loaded and operational.
5.4. Preliminary Set-Up
5.4.1 Ensure that the mobile phase and sample vials are properly labeled and ready for use.
5.4.2 Perform any necessary column equilibration or conditioning, following method-specific instructions.
6. HPLC System Shutdown
6.1. Completing the Analysis
6.1.1 Finish the analysis as per the established chromatographic method.
6.1.2 Ensure that all data is saved properly.
6.2. Shutting Down the System
6.2.1 Stop the flow of the mobile phase.
6.2.2 Power off the HPLC system.
6.2.3 Properly store any columns or equipment not in use.
6.3. Post-Analysis Checks
6.3.1 Verify that all waste containers are properly labeled and ready for disposal.
6.3.2 Clean the system, including sample injection ports and detectors, following established procedures.
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