Standard Operating Procedure (SOP) for Proper Sample Preparation: Dilution, Extraction, and Filtration

Standard Operating Procedure (SOP) for Cleaning of U.V. / Visible Spectrophotometer

1. Purpose of Proper Sample Preparation: Dilution, Extraction, and Filtration

The purpose of this Standard Operating Procedure (SOP) for Proper Sample Preparation: Dilution, Extraction, and Filtration is to establish a systematic process for proper sample preparation, including dilution, extraction, and filtration, at [Company Name]. This procedure ensures that samples are accurately and consistently prepared for analytical testing.

2. Scope of Proper Sample Preparation: Dilution, Extraction, and Filtration

This SOP for Proper Sample Preparation: Dilution, Extraction, and Filtration applies to all personnel responsible for sample preparation within [Company Name]. It covers the processes of dilution, extraction, and filtration, which may be used in various laboratory applications.

3. Definitions involved in Proper Sample Preparation: Dilution, Extraction, and Filtration

3.1 Sample:

A representative portion of a material to be analyzed.

3.2 Dilution:

The process of reducing the concentration of a sample by adding a suitable solvent.

3.3 Extraction:

The process of removing specific components from a sample.

3.4 Filtration:

The separation of solids from liquids by passing the sample through a filter medium.

4. Responsibilities involved in Proper Sample Preparation: Dilution, Extraction, and Filtration

4.1. Laboratory Manager

4.1.1 Ensure that all sample preparation procedures are performed in compliance with this SOP.

4.1.2 Allocate resources and personnel for sample preparation activities.

4.2. Sample Preparation Technician

4.2.1 Perform sample preparation activities, including dilution, extraction, and filtration, as required.

4.2.2 Ensure that all procedures are followed accurately and consistently.

5. Sample Dilution

5.1. Pre-Dilution Preparations

5.1.1 Identify the sample to be diluted and determine the required dilution factor.

5.1.2 Select an appropriate diluent that is compatible with the sample and the intended analysis.

5.2. Dilution Procedure

5.2.1 Add the calculated volume of sample to an appropriate container.

5.2.2 Add the calculated volume of diluent to the same container.

5.2.3 Mix the contents thoroughly to achieve homogeneity.

6. Sample Extraction

6.1. Pre-Extraction Preparations

6.1.1 Determine the components to be extracted and select the appropriate extraction solvent.

6.1.2 Prepare extraction equipment and containers.

6.2. Extraction Procedure

6.2.1 Add the sample to an extraction container.

6.2.2 Add the extraction solvent to the container.

6.2.3 Agitate or mix the sample and solvent as necessary.

6.2.4 Allow the extraction to proceed for the specified duration.

6.2.5 Separate the extracted liquid from any solid or particulate matter.

7. Sample Filtration

7.1. Pre-Filtration Preparations

7.1.1 Select the appropriate filter medium (e.g., paper, membrane) and filtration equipment.

7.1.2 Ensure that filtration equipment is clean and properly assembled.

7.2. Filtration Procedure

7.2.1 Place the filter medium in the filtration apparatus.

7.2.2 Add the extracted sample to the filter apparatus.

7.2.3 Apply vacuum or pressure as needed to facilitate filtration.

7.2.4 Collect the filtrate in a clean container.

8. Record Keeping

8.1. Sample Preparation Records

8.1.1 Maintain detailed records for each sample preparation step, including dilution, extraction, and filtration.

8.1.2 Include information about sample identification, dates, and personnel involved.

8.2. Schedule

8.2.1 Establish a schedule for sample preparation activities as needed.

8.2.2 Ensure that the schedule is consistently followed.

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